Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1984-08-30
1993-02-16
Saunders, David A.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
436536, 436537, 436800, G01N 33536, G01N 33542
Patent
active
051871068
ABSTRACT:
A fluorescence decay time method and apparatus directly measuring the time decay function resulting from a plurality of fluorescing bodies, including background, and analytically determines the presence and concentration of the fluorescing bodies homogeneously and simultaneously is disclosed.
The fluorescently labelled reaction product of a target ligand, such as an antigen, with a binding molecule for the ligand, such as an antibody, exhibits a fluorescent decay time curve which bears a direct straight line relationship to the concentration of the target antigen, in that the value for fluorescent intensity at time zero for the decay time curve is a measure of said concentration.
The time zero intensity is proportional to the concentration of the target ligand in the unknown sample which can then be compared to the concentrations from a standard curve.
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patent: 4058732 (1977-11-01), Wieder
patent: 4352751 (1982-10-01), Wieder et al.
E. Soini et al Clin. Chem. 25, 353-361, 1979.
F. J. Knorr et al Anal. Chem., 53, 272-276, 1981.
F. Komen et al, Steroids, 36, 421-427, 1980.
Fritzsche Robert W.
Schlager Kenneth J.
Orbit Medical Systems, Inc.
Saunders David A.
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