Methods for determination of enzyme reactions

Details Methods for determination of enzyme reactions Methods for determination of enzyme reactions

1976-07-28

1978-04-18

Tung, T.

Chemistry: electrical and wave energy

Processes and products

204195B, 1951035R, G01N 2746

Patent

active

040850099

ABSTRACT:
A method, for microcoulometric determination either of the activity of an enzyme in a liquid sample or the concentration of a substrate for a reaction involving a change in a coenzyme from a first form thereof to a second form. At intervals during the reaction, both coenzyme forms are measured essentially simultaneously utilizing an electrolytic cell, to indicate either such enzyme activity or substrate concentration, depending upon which is the unknown factor.

REFERENCES:
patent: 3242064 (1966-03-01), Byrne
patent: 3654116 (1972-04-01), Inoue
patent: 3765841 (1973-10-01), Paulson
patent: 3838034 (1974-09-01), Groves
patent: 3857771 (1974-12-01), Sternberg
patent: 4016044 (1977-04-01), Fresnel et al.
patent: 4045297 (1977-08-01), Weeks et al.
Thomas et al., "Analytica Chemica Acta", vol. 78, 1975, pp. 271-276.

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