Chemistry: electrical and wave energy – Apparatus – Electrolytic
Patent
1995-06-07
1997-03-04
Bell, Bruce F.
Chemistry: electrical and wave energy
Apparatus
Electrolytic
204403, 204415, 204416, 4352871, 435817, 435 24, 422 8203, 2057775, 2057895, 2057925, G01N 2726
Patent
active
056075677
ABSTRACT:
A polymeric membrane electrode gives potentiometric responses to macromolles, such as protamine, in solution or biological specimens. A protamine-responsive membrane includes a lipophilic cation-exchange material for ionic macromolecules in a specially formulated plasticized, polymeric matrix material. Potentiometric response to protamine has been observed in membranes comprising 30-70 wt. % polymeric matrix material, such as polyvinyl chloride; 1-3 wt. % salt of a tetraphenylborate, such as potassium tetrakis(4-chlorophenyl) borate or salt of an organophosph(on)ate, such as calcium bis-[4-(1,1,3,3-tetramethylbutyl)phenyl] phosphate; and 30-70 wt. % of a plasticizer, such as 2-nitrophenyl octyl ether or tris (2-ethylhexyl) phosphate. The potentiometric response of these membranes to protamine can be used to study the binding interaction of protamine with heparin (both native and low molecular fractions) or to monitor protease activity, for example, by following the rate of degradation of intact protamine or a synthetic substrate by trypsin, as well as to measure protamine concentration in biological fluids, such as blood or plasma.
REFERENCES:
patent: 3932233 (1976-01-01), Ruzicka et al.
patent: 4770759 (1988-09-01), Young et al.
patent: 5236570 (1993-08-01), Ma et al.
patent: 5401377 (1995-03-01), Shieh et al.
patent: 5417835 (1995-05-01), Brown
Meyerhoff Mark E.
Yang Victor C.
Yun Jong H.
Bell Bruce F.
The Board of Regents Acting for and on behalf of University of M
LandOfFree
Protamine-responsive polymeric membrane electrode does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Protamine-responsive polymeric membrane electrode, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Protamine-responsive polymeric membrane electrode will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2143704