Detection of antibodies to bacterial antigens by flourescence po

Details Detection of antibodies to bacterial antigens by flourescence po Detection of antibodies to bacterial antigens by flourescence po

1995-08-28

1999-11-02

Caputa, Anthony C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 71, 435 72, 436536, 530300, 530322, 530350, G01N 33554, G01N 33569, G01N 3353, C07K 100

Patent

active

059768207

ABSTRACT:
In a homogeneous immunoassay, fluorophore-conjugated lipopolysaccharide derived bacterial antigens are reacted with antibodies specific for the antigens. Quantitative detection of the formation of an immune complex is obtained by measuring the change in fluorescence polarization after complex formation. The reaction occurs quickly (less than two minutes to equilibrium), and involves the addition of only one reagent to a diluted serum specimen. The absence of a solid phase separation step eliminates false positive results and increases throughput.

REFERENCES:
patent: 4831126 (1989-05-01), Bundle et al.
patent: 4921878 (1990-05-01), Rothman et al.
patent: 5212298 (1993-05-01), Rademacher et al.
patent: 5445820 (1995-08-01), Seidel et al.
patent: 5445935 (1995-08-01), Royer
Lazar et al (Molecular & Cellular Biology vol. 8 No. 3 pp. 1247-1252), Mar. 1988.
Burgess et al (J. of Cell Biology vol. 111 pp. 2129-2138), Nov. 1990.
Salgaller et al (Cancer Immuno. Immunother. (39) pp. 105-116), 1994.
Toomre et al (Glycobiology vol. 4 No. 5 pp. 653-663), 1994.
Miller et al (Am. J. Trop. Med. Hyg. 32 (3) pp. 555-564), 1983.

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