Neisseria gonorrhoeae specific DNA fragment--GC3

Details Neisseria gonorrhoeae specific DNA fragment--GC3 Neisseria gonorrhoeae specific DNA fragment--GC3

1998-04-27

1999-11-02

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 231, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

059768053

ABSTRACT:
Disclosed herein is a newly-identified DNA sequence from Neisseria gonorrhoeae designated GC3. Also disclosed are methods, oligonucleotide probes, amplification primers, and kits for the species-specific detection of N. gonorrhoeae. Preferably, N. gonorrhoeae is detected by amplifying the N. gonorrhoeae nucleic acids using the disclosed amplification primers, and then detecting the amplified nucleic acids. In a more preferred embodiment, N. gonorrhoeae nucleic acids are amplified and detected by thermophilic strand displacement amplification (tSDA). Also preferred are methods for detecting N. gonorrhoeae that are employed in conjunction with methods for detecting other sexually-transmitted pathogens, in particular, Chlamydia trachomatis.

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