Enhancement of lipid cationic transfections in the presence of s

Details Enhancement of lipid cationic transfections in the presence of s Enhancement of lipid cationic transfections in the presence of s

1994-10-27

1997-05-06

Campbell, Bruce R.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

424450, 4351721, 4351723, 935 52, 935 54, A61K 4800, A61K 9127, C12N 500

Patent

active

056271590

ABSTRACT:
A method is provided for enhancing transfection efficiency of eukaryotic cells comprising contacting said cells with a lipid aggregate comprising nucleic acid and a polycationic lipid composition in the presence of a polycationic compound and serum. The polycationic compound is preferably Polybrene.TM., and the lipid aggregate preferably comprises liposomes of nucleic acid and LipofectAMINE.TM..

REFERENCES:
Brunette et al (1992) Nucleic Acids Research 20:1151.
Zhou et al (1991) Biochimica et BioPhysica Acta 1065:8-14.

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