Kit for in vitro cell growth of eucaryotes using low molecular w

Details Kit for in vitro cell growth of eucaryotes using low molecular w Kit for in vitro cell growth of eucaryotes using low molecular w

1997-08-14

1999-03-23

Naff, David M.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Culture medium, per se

435252, 435348, 435366, 435325, C12N 122

Patent

active

058858350

ABSTRACT:
A method and kit are provided for growing eucaryotic cells using a hydrolyzate of a protein material containing peptides and free amino acids. The hydrolyzed protein material contains L-glutamine, preferably in an amount of greater than 20 percent by weight. Ninety percent by weight of the hydrolyzed protein material is less than 1,000 kD in molecular weight. The free amino acid level is less than 20 percent by weight and the average peptide length is less than 20 amino acids.

REFERENCES:
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patent: 4235772 (1980-11-01), Lunden et al.
Heeneman et al, J. Immunological Methods, 116, 85-91 (1991).
Animal Cell Culture, A Practical Approach, Second Edition, ISBN 0-19-963213-8.
MacRitchie, J. Food Technol. 14, 595,601 (1979).
Brand, K., et al., Metabolism 38:29-33 (1989).
Tanabe et al., Journal of Food Biochemistry, 16:235-248 (1993).

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