Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1998-05-22
1999-03-23
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 15, 435 23, 435 26, 435 14, C12Q 100, C12Q 148, C12Q 137, C12Q 154
Patent
active
058857672
ABSTRACT:
A method for quantitating L-homocysteine and/or L-methionine in a solution involves contacting a solution containing L-homocysteine and/or L-methionine with a reagent comprising methionine gamma-lyase and a cofactor capable of forming a Schiff base with the L-methionine and/or L-homocysteine for a time sufficient to catalyze the conversion of L-homocysteine and/or L-methionine to 2-ketobutyrate. The amount of 2-ketobutyrate formed is determined, and the amount of L-homocysteine and/or L-methionine present in the original solution can be determined based on the amount of 2-ketobutyrate formed. A composition for measuring the amount of L-homocysteine and/or L-methionine in a solution comprises methionine gamma-lyase, a cofactor capable of forming a Schiff base with the L-methionine and/or L-homocysteine and at least one 2-ketobutyrate detecting agent, but is substantially free of L-methionine, L-homocysteine, 2-ketobutyrate, pyruvate and mercury.
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BioCatalytics, Inc.
Leary Louise N.
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