Preparation of polymeric thiol gels for covalent bonding of biol

Details Preparation of polymeric thiol gels for covalent bonding of biol Preparation of polymeric thiol gels for covalent bonding of biol

1987-10-13

1989-12-12

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435178, 435180, 435181, 435814, 436529, 436531, 436532, 525 541, 530813, 530815, 530816, 536 11, 536 54, C12N 900, C12N 1106, G01N 33547, C07K 1706

Patent

active

048867551

ABSTRACT:
Thiol gels are prepared from hydroxyl containing polymers by forming a 2-fluoro-1-methylpyridoxy derivative of the polymer followed by reacting the derivative with sodium dimethyldithiocarbamate and reducing to produce a sulfhydryl substituted polymer. Alternatively, the derivative is reacted with dithiothreitol to produce a sulfhydryl substituted polymer having free sulfhydryl groups spaced from the polymer. The thiol gel can be activated by means of 2,2'-dipyridyl disulfide and reacted with a free sulfhydryl containing biologically active ligand in order to provide an insolubilized ligand which can be used for various purposes including use as an immobilized biologically active material or use as a matrix in covalent chromatography.

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