Method for classifying and counting leukocytes

Details Method for classifying and counting leukocytes Method for classifying and counting leukocytes

1992-06-23

1994-05-03

Housel, James C.

Chemistry: analytical and immunological testing

Biological cellular material tested

436 17, 436172, 436174, 436800, G01N 3348

Patent

active

053087720

ABSTRACT:
A hematological specimen for classifying and counting leukocytes with a flow cytometer is prepared. A sample to be assayed is prepared by eliminating influences of erythrocytes from a hematological sample without changing leukocytes morphologically; adjusting the pH value to a level suitable for staining; and staining the leukocytes with at least two dyes including Astrazon Yellow 3G capable of specifically staining at least basophils and immature granulocytes and Neutral Red capable of specifically staining at least eosinophils. Thus leukocytes contained in the hematological sample can be classified at least three or six groups including immature granulocytes by measuring a single specimen with a flow cytometer.

REFERENCES:
patent: 3906120 (1975-09-01), Geating
patent: 4751179 (1988-06-01), Ledis et al.
patent: 5039613 (1991-08-01), Matsuda et al.
patent: 5122453 (1992-06-01), Martin et al.
patent: 5175109 (1992-12-01), Sakata et al.
Frankel et al., "Gradwohl's Clinical Laboratory Method & Diagnosis", 1970, p. 508.

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