Multi-cellular, three-dimensional living mammalian tissue

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4352402, 43524025, 435 1, 435286, 435312, C12N 500, C12N 502, A01N 102, C12M 302, C12M 110

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active

053087640

ABSTRACT:
The present invention relates to a multicellular, three-dimensional, living mammalian tissue. The tissue is produced by a co-culture process wherein two distinct types of mammalian cells are co-cultured in a rotating bioreactor which is completely filled with culture media and cell attachment substrates. As the size of the tissue assemblies formed on the attachment substrates changes, the rotation of the bioreactor is adjusted accordingly.

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Goodwin et al, In Vitro Cell. Dev. Biol., 28A (Jan. 1992) pp. 47-60.
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Knuechel et al, J. of Urology, 139(3), 1988, pp. 640-645 (Biosis Abstract #85113082).

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