Homogeneous enzyme assay for antibodies

Details Homogeneous enzyme assay for antibodies Homogeneous enzyme assay for antibodies

1977-06-02

1980-02-26

Tanenholtz, Alvin F.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

G01N 3114

Patent

active

041904966

ABSTRACT:
Novel biological assay method for determining the presence of a specific organic material by employing a modified enzyme for amplification. By employing receptors specific for one or a group of materials (hereinafter referred to as "ligands") and binding an enzyme to the ligand or ligand counterfeit to provide an "enzyme-bound-ligand", an extremely sensitive method is provided for assaying for ligands. The receptor when bound to the enzyme-bound-ligand substantially inhibits enzymatic activity, providing for different catalytic efficiencies of enzyme-bound-ligand and enzyme-bound-ligand combined with receptor.
The receptor, ligand and enzyme-bound-ligand are combined in an arbitrary order and the effect of the presence of ligand on enzymatic activity determined. Various protocols may be used for assaying for enzymatic activity and relating the result to the amount of ligand present.
The subject method may also be used for determining receptors, employing the same procedure, except for not including receptor as a reagent.

REFERENCES:
patent: 3654090 (1972-04-01), Schuurs et al.

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