Methods for attaching polynucleotides to supports

Details Methods for attaching polynucleotides to supports Methods for attaching polynucleotides to supports

1985-06-13

1989-01-10

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 935 77, 935 78, C12Q 168, G01N 33566

Patent

active

047973552

ABSTRACT:
A probe is attached to a stock support to which is bound a single-stranded polynucleotide by hybridizing both the probe and the bound polynucleotide to a connecting strand with which they are both complementary and then ligating an end of the bound polynucleotide and the probe. After denaturation, the probe remains attached to the support by way of the bound polynucleotide. A target polynucleotide is then hybridized with the support-bound probe and with a labelled probe to provide a detectable hybridization complex.

REFERENCES:
patent: 4358535 (1982-07-01), Falkow et al.
patent: 4415732 (1983-03-01), Caruthers et al.
Cozzarelli, et al., Biochem. Biophys. Res. Comm. 28, 578-586 (1967).
Dunn, et al., Cell, 12, 23-36 (1977).
Maniatis, et al., Molecular Coning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York (1982) (Standard Text Not Enclosed).
Ranki, et al., Gene, 21, 77-85 (1983).
Thomas, Proc. Natl. Acad. Sci. (U.S.A.), 77, 5201-5205 (1980).
Wu et al., Proc. Natl. Acad. Sci. (U.S.A.), 78 7059-7063 (1981).

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