Polypeptide

Details Polypeptide Polypeptide

1983-07-28

1986-05-20

Apley, Richard J.

Prosthesis (i.e., artificial body members), parts thereof, or ai

Implantable prosthesis

623 66, 128 1R, 2601125R, 530350, 530815, A61F 100, A61K 3700, C07C10352, C07G 700

Patent

active

045898812

ABSTRACT:
A polypeptide having the cell-attaching activity of fibronectin. The polypeptide has 108 amino acid residues and the formula: H-Ile-Gly-Gln-Gln-Ser-Thr-Val-Ser-Asp-Val-Pro-Arg-Asp-Leu-Glu-Val-Val-Ala- Ala-Thr-Pro-Thr-Ser-Leu-Leu-Ile-Ser-Trp-Asp-Ala-Pro-Ala-Val-Thr-Val-Arg-Tyr -Tyr-Arg-Ile-Thr-Tyr-Gly-Glu-Thr-Gly-Gly-Asn-Ser-Pro-Val-Gln-Glu-Phe-Thr-Va l-Pro-Gly-Ser-Lys-Ser-Thr-Ala-T r-Ile-Ser-Gly-Leu-Lys-Pro-Gly-Val-Asp-Tyr-Thr-Ile-Thr-Val-Tyr-Ala-Val-Thr- Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu -Ile-Asp-Lys-Pro-Ser-Gln-Met-OH. The polypeptide or a biologically active fragment thereof, such as H-Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile- Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-OH can be employed in the preparation of substrata designed for the attachment of cells thereto. A Cys-residue may optionally be attached at the C-terminus. It can be linked to the surface of a prosthetic device to particularly attract endothelial cells and fibroblastic cells.

REFERENCES:
Pierschbacher et al.; "The Cell Attachment . . . "; The Journal of Bio. Chem.; vol. 257, No. 16, pp. 9593-9595; 8/25/82.
Petersen et al.; "Partial Primary Structure . . . "; Proc. Natl. Acad. Sci.; vol. 80, pp. 137-141; 1/1983.
Hynes, et al., Cell Surface Fibronectin and Oncogenic Transformation, J. Supramolecular Structure, 11:95-104, 1979.
Hahn, et al., Isolation and Biological Characterization of Active Fragments of the Adhesive Glycoprotein Fibronectin, Cell, 18:1043-1051, 1979.
Sekiguchi, et al., Functional Domain Structure of Fibronectin, Proc. Natl. Acad. Sci., 77:2661-2665, 1980.
Grinnell, Frederick, et al., Distribution of Fibronectin during Wound Healing in Vivo., J. Invest. Dermatol., 76:181-189, 1981.
McDonagh, R. P., et al., Amino Acid Sequence of the Factor XIII.sub.a Acceptor Site in Bovine Plasma Fibronectin, Febs Letters, 127:174-178, 1981.
Ruoslahti, Erkki, et al., Alignment of Biologically Active Domains in the Fibronectin Molecule, J. Biol. Chem., 256:7277-7281, 1981.
Pande, Hema, et al., Comparative Structural Studies of Human Plasma and Amniotic Fluid Fibronectins, Bioch. Biophy. Res. Comm., 101:265-272, 1981.
Pande, Hema, et al., NH.sub.2 -Terminal Sequences of DNA-, Heparin-, and Gelatin-Binding Tryptic Fragments from Human Plasma Fibronectin, Arch. Bioch. Biophy., 213:258-265, 1982.
Seitz, T. L., et al., Effect of Fibronectin on the Adhesion of an Established Cell Line to a Surface Reactive Biomaterial, J. Biomed. Mat. Res., 16:195-207, 1982.
Vibe-Pedersen, Karen, et al., Amino Acid Sequence of a Peptide from Bovine Plasma Fibronectin Containing a Free Sulfhydryl Group (Cysteine), Febs. Letters, 142:26-30, 1982.
Sekiguchi, Kiyotoshi, et al., Monoclonal Antibodies Directed to Two Different Domains of Human Plasma Fibronectin: Their Specificities, Fed. Eur. Bich. Soc., 142:243-246, 1982.
Ehrismann, Ruth, et al., Arrangement of Attachment-Promoting, Self-Association, and Heparin-Binding Sites in Horse Serum Fibronectin, J. Biol. Chem., 257:7381-7387.
Clark, Richard A. F., et al., Fibronectin is Produced by Blood Vessels in Response to Injury, J. Exp. Med., 156:646-651, 1982.
Hynes, Richard O., et al., Fibronectins: Multifunctional Modular Glycoproteins, J. Cell. Biol., 95:369-377, 1982.
Petersen, Torbene, et al., Partial Primary Structure of Bovine Plasma Fibronectin: Three Types of Internal Homology, Proc. Natl. Acad. Sci., 80:137-141, 1983.
Frederick Grinnell, Cell Attachment and Spreading Factors, in Growth and Maturation Factors, (Dr. Gordon Guroff, Ed.) John Wiley & Sons, Inc., 1983.
Grinnell, Frederick, The Role of Fibronectin in the Bioreactivity of Material Surfaces in Biocompatible Polymers, Metal, and Composites, (Ed. Michael Szycher), Technomic Publishing, Lancaster, Pennsylvania, 1983.
Hayashi, Masao, et al., Domain Structure of the Carboxyl-Terminal Half of Human Plasma Fibronectin, J. Biol. Chem., 258:3332-3340, 1983.
Kornblihtt, Alberto R., et al., Isolation and Characterization of cDNA Clones in Human and Bovine Fibronectins, Proc. Natl. Acad. Sci., 80:3218-3222, 1983.

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