Methods for the detection and determination of ligands

Details Methods for the detection and determination of ligands Methods for the detection and determination of ligands

1978-09-21

1980-10-14

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

23230B, 424 12, 435188, C12Q 166

Patent

active

042282373

ABSTRACT:
A method for determining the presence of a ligand in a liquid medium which utilizes enzyme labeled avidin and biotin labeled reagent in a specific binding process wherein the ligand to be detected is contacted with an insoluble phase containing specific binding substance for the ligand. Various protocols may be used to assay the resulting enzyme activity which is related to the amount of ligand in the liquid medium.

REFERENCES:
patent: 4002532 (1977-01-01), Weltman et al.
patent: 4016043 (1977-04-01), Schums et al.
patent: 4134792 (1979-01-01), Boguslnski et al.
May, et al., "N.sup..epsilon.B29 -(+)-Biothylinsulin and its Complexes with Audin", J. Biol Chem., vol. 253, No. 3, (1978) pp. 686-690.
Manning, et al., "A Method for Gene Enrichment Based on the Auidin-Biochem Interaction Application to the Drosophilia Ribosomul Genes," Biochem., vol. 16, No. 7 (1977), pp. 1364-1370.
Heggeness, et al., "Use of the Avidin-Biotin Complex for the Localization of Actin and Myosin with Fluorescene Microscopy," J. Cell Biol. vol. 73 (1977), pp. 783-788.
Hofmann, et al., "Avidin-Biotin Affinity Columns, General Methods for Attaching Biotin to Peptides and Proteins," J. Am. Chem. Soc. vol. 100, No. 11 (1978), pp. 3585-3590.
Bayer, et al., "Preparation of Ferritin-Avidin Conjugates by Reductive Alkylation for Use in Electron Microscope Cytochemistry," J. Histochem and Cytochem, vol. 24, No. 8, (1976), pp. 933-939.
Wisdom, "Enzyme-Immunoassay," Clin. Chem., vol. 22 No. 8, (1976), pp. 1243-1255.

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