Balanced inducible transcription system

Details Balanced inducible transcription system Balanced inducible transcription system

1991-06-21

1993-11-30

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

43525233, 4351723, C12N 1500, C12N 964, C12N 121

Patent

active

052664740

ABSTRACT:
A balanced constitutive inducible transcriptional control system is provided to facilitate the expression of polypeptides incompatible with recombinant host cells. Use of this system has, for the first time, made it possible to produce mature, unglycosylated human tissue plasminogen activator in prokaryotes which is water soluble and fully enzymatically active. In accordance with this invention, t-PA is produced by recombinant bacterial host cell culture in commercially significant amounts without in vitro renaturation and processing.

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