Method of preparing immunological analytical element

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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422 56, 422 57, 422 58, 435 792, 435969, 435970, C12Q 125, G01N 33535

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active

052664600

ABSTRACT:
A method of preparing a dry-type analytical element for immunoassay having at least one water-permeable porous layer and containing a labeled antigen and an antibody to said labeled antigen and to an unlabeled antigen as the components participating in antigen-antibody reaction in said porous layer, which comprises applying or impregnating a first composition containing one of said labeled antigen and said antibody and thereafter applying or impregnating a second composition containing the other of said labeled antigen and said antibody dissolved or suspended in a solvent not dissolving substantially said labeled antigen or said antibody in the first composition onto said porous layer.

REFERENCES:
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4042335 (1977-08-01), Clement
patent: 4168146 (1979-09-01), Grubb et al.
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4447529 (1984-05-01), Greenquist et al.

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