Method of purifying alcohol dehydrogenase

Details Method of purifying alcohol dehydrogenase Method of purifying alcohol dehydrogenase

1977-11-02

1978-09-05

Shapiro, Lionel M.

Textiles: fiber preparation

526 50, 536112, C07G 7026

Patent

active

041117514

ABSTRACT:
An affinity resin containing pyrazole immobilized on a solid insoluble support by a coupling arm bonded at the 4-position having a length of at least 12 Angstrom units is used to purify and fractionate crude alcohol dehydrogenase extract into a pyrazole-binding fraction and a pyrazole-insensitive fraction containing the anodic band by bringing the resin into contact with an aqueous solution of a binary complex of alcohol dehydrogenase and NAD to bind the binary complex, then eluting the complex. The bound fraction can be further fractionated into isoenzymes during elution by adjusting the alcohol gradient of the eluant, and the anodic band can be separated from the remainder of the solution, which does not bind to pyrazole, by salting out and chromatography on agarose-AMP resin.

REFERENCES:
patent: 3912595 (1975-10-01), Philipp et al.
patent: 3920631 (1975-11-01), Molteni et al.
patent: 3926730 (1975-12-01), Huper et al.
patent: 3951744 (1976-04-01), Roder et al.
patent: 3970603 (1976-07-01), Gray
patent: 4030997 (1977-06-01), Fujii et al.
Li et al., Biochem. Biophys. Res. Comm. 63, 202-208 (1975).

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