Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1989-09-29
1992-12-15
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, 435968, C12Q 128, C12Q 126
Patent
active
051716682
ABSTRACT:
A method of chemiluminescence assay of the activity of peroxidase is disclosed, which employes (i) luminol or isoluminol as a substrate, (ii) hydrogen peroxide, and (iii) at lease one compound, serving as an enhancer for the activity of peroxidase, selected from the group consisting of ##STR1## wherein R.sup.1 represents a hydroxyl group, an alkali metal salt of hydroxyl group (--OM, where M is an alkali metal), an amino group which may have a substituent, a halogen, an alkyl group having 1 to 4 carbon atoms, which may have a substituent; R.sup.2 and R.sup.3 each represent hydrogen or a halogen; R.sup.4 and R.sup.5 each represent hydrogen, an alkyl group having 1 to 4 carbon atoms, which may have a substituent, or an alkoxyl group having 1 to 4 carbon atoms, which may have a substituent; ##STR2## wherein R.sup.6 represents an alkyl group having 1 to 6 carbon atoms, which may have a substituent, or an alkene group having 2 to 6 carbon atoms, which may have a substituent, R.sup.7 to R.sup.10 each represent hydrogen, an alkyl group having 1 to 6 carbon atoms, which may have a substituent, or a halogen, X represents oxygen or sulfur, Y represents --CH-- or nitrogen, which may be the same or different, and Y' represents carbon or nitrogen, which may be the same or different.
REFERENCES:
patent: 4729950 (1988-03-01), Kricka et al.
Bodea et al., "Recent Advances in the Chemistry of Phenothiazines", Advances in Heterocyclic Chemistry, vol. 9, Academic Press, N.Y., pp. 356-368, 389-393 (1968).
Thorpe et al., Meth. Enzymol., vol. 133, Academic Press, N.Y., pp. 331-353 (1986).
Pinder et al., Meth. Enzymol., vol. XVIII, Academic Press, N.Y., pp. 24-30.
Chemical Abstracts, vol. 108, No. 21, May 23, 1988, p. 367, Abstract No. 183150O, Columbus, Ohio, U.S.: M. A. Motsenbocker: "Sensitivity Limitations Encountered in Enhanced Horseradish Peroxidase Catalyzed Chemiluminescence", & J. Biolumin. Chemilumin., 1988, 2(1), 9-16.
Fujirebio Inc.
Nucker Christine M.
Woodward M. P.
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