Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Patent
1986-07-08
1988-08-30
Rosen, Sam
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
514 2, 435243, 435245, 435853, A61K 3700
Patent
active
047676230
DESCRIPTION:
BRIEF SUMMARY
The present invention relates to a method for microbial colonisation of the oesophagus, stomach and intestines of animals and humans by utilizing bacteria beneficial to the host organism. The invention also relates to a method strengthening the mechanism for adhesion of desired species of bacteria applied. The invention also relates to preparations for achieving the results mentioned above.
The invention will be described and is applicable for both animals and humans. "Animals" here relates to domestic animals such as pigs, calves and poultry, such as chickens, turkeys, geese and ducks.
At present the mortality rate amongst the above-mentioned animals is high, and is caused by the establishment and colonisation of pathogenic bacteria in the stomach and intestines. Pathogenic bacteria out compete the normal bacteria flora, adhere to the wall of the intestines and give rise to symptoms of disease such as diarrhoea, and results in increased mortality. The losses in some herds or flocks are considerable as a result of outbreaks of pathogenic bacteria as Escherichia coli, Salmonella typhimurium, Salmonella sp., Shigella sp. and Clostridium perfringens.
Numerous attempts are currently being made to introduce nonpathogenic bacteria into the gastro intestinal tract in order to prevent or remove colonisation of pathogenic bacteria. Oral administration of different types of bacillus to humans has been found to reduce the risk of cancer in the large intestine and increase the removal or ousting of intestinal pathogens as well as being of therapeutic value of elderly patients. Equivalent studies of animals and humans have the common factor that bacteria introduced in the gastro intestinal tract in order to improve the state of health and increase survival in the host organism. Colonisation of these bacteria is possible provided the bacteria cells are capable of attaching themselves to the epithelium walls of the host organism.
An almost continuous supply of large bacteria cultures is required to achieve the above results, which makes the method impractical.
Most studies of the above type have been carried out on animals and show negative results with respect to adhesion and colonisation of the specific type of bacteria used. Existing methods have the following problems, which also prevent successful experiments: selective colonisation or survival. investigated and determined in in vitro experiments. The limitation here is that the mechanism for adhesion is not defined and specific (lectinmediated) binding cannot be determined.
Some examples illustrate these limitations:
In pigs: manner. administered orally. Colonisation was not obtained in either case.
In humans: initially showed favourable results, but without colonisation being obtained. The results usually disappeared after 3 days, a decline which accords well with the reduction in the number of Lactobacillus cells in the host organism.
In research into the adhesion of lactic acid bacteria e.g. the genera Lactobacillus and Streptococcus to the gastro intestinal tract in laboratory animals it was found contrary to accepted belief, that the specific binding of the bacteria to epithelial cells in the host organism, is not mediated by polysaccharides, but rather by a protein.
The following points constitute the bases of the process on which the present invention is founded: genera Lactobacillus and Streptococcus to the oesophagus and gastro intestinal tract consists of an extracellular protein in the following referred to as adhesive promoting protein (APP) on the bacteria surface. bacteria thus increasing the adhesion of bacteria cells. administering can be modified to give maximum production of APP. bacterialstrains to be used for colonising the oesophagus and/or gastro intestinal tract.
The invention is illustrated further with reference to the following non-limiting examples, relating to experiments with animals.
EXAMPLE 1
The cultivation conditions, relating to harvesting and adhesion capacity of lactic acid bacteria were investigated. The adhesion was determin
REFERENCES:
patent: 3320130 (1967-05-01), Henry
patent: 3713836 (1973-01-01), Carlsson et al.
patent: 4314995 (1982-02-01), Hata et al.
patent: 4332790 (1982-06-01), Sozzi et al.
Livsmedels Teknik, vol. 24, No. 9, pp. 434-436.
Japanese Patents Report, vol. 84, No. 48, 1985, (Derwent Publications Ltd, London), Section CH: Chemical, J8-C, No. J8 4 046 209-B, pub. 1984-11-10.
Conway Patricia
Kjelleberg Staffan
Chemical Dynamics Sweden AB
Rosen Sam
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