Integral element for the analysis of cholesterol

Details Integral element for the analysis of cholesterol Integral element for the analysis of cholesterol

1975-04-07

1976-09-28

Monacell, A. Louis

Textiles: fiber preparation

195127, C12K 104

Patent

active

039830055

ABSTRACT:
An integral analytical element for analysis of liquids for their cholesterol content is disclosed. The element is of the type which comprises at least two superposed layers including a spreading layer and a reagent layer in fluid contact and, optionally, a support. Cholesterol oxidase and a composition for the hydrolysis of cholesterol esters comprising lipase having cholesterol esterase activity and protease are included in the element such that cholesterol esters contained in a sample applied to the spreading layer are saponified to free cholesterol and free cholesterol is decomposed in the presence of cholesterol oxidase to produce a detectable change related to the total cholesterol content of the sample.

REFERENCES:
patent: 3298789 (1967-01-01), Mast
patent: 3607093 (1971-09-01), Stone
patent: 3798004 (1974-03-01), Zerachia et al.
patent: 3802842 (1974-04-01), Lange et al.
patent: 3847553 (1974-11-01), Verbeck
"Method and Composition for Blood Serum Cholesterol Analysis" Research Disclosure vol. 127 pp. 39-42 (1974).

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