Mycobacteria culture medium and method for in vitro cultivation

Details Mycobacteria culture medium and method for in vitro cultivation Mycobacteria culture medium and method for in vitro cultivation

1976-01-20

1976-09-28

Monacell, A. Louis

Textiles: fiber preparation

195100, C12B 314, C12B 100

Patent

active

039830039

ABSTRACT:
A hyaluronic acid-enriched mycobacteria culture medium whose base is either Dubos oleic acid-albumin liquid medium or a physiological mixture of fresh yeast extract in a phosphate buffer of pH 5.5 to 7 optionally containing bovine serum albumin, glycerine, a contaminating organism-growth inhibitory antibiotic, and a gelatinizing agent to solidify the culture medium for plate use. The culture medium has particular utility for the in vitro cultivation of leprosy mycobacteria, which exhibit prolific growth when inoculated and maintained in such culture medium at temperatures within the range of about 25.degree.C to about 37.5.degree.C.

REFERENCES:
buchanan et al., Bergey's Manual of Determinative Bacteriology, The Williams and Wilkins Company, Baltimore, 8th ed., (1974) pp. 682, 683, 700, 701.
Olitzki et al., "Effect of Inorganic and Organic Substances Containing Sulfur or Nitrogen and of Vitamins on the Growth of Mycobacterium Leprae," Chemical Abstracts, vol. 81, No. 13, p. 199 (1974).
Nakamura, "Multiplication of Mycobacterium Lepraemurium in Cell-free Medium," Chemical Abstracts, vol. 78, No. 25, pp. 248, 249 (1973).
Dhople et al., "Energetics (adenosine 5'-triphosphate) of Mycobacterium Lepraemurium in Diffusion Chambers Incubated in Vitro and in Mice," Chemical Abstracts, vol. 80, No. 13, p. 151 (1974).

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