Method for preparing single cell suspension

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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435803, 210460, 210927, 241 30, 2411692, C12N 502, B01D 2900, B26B 2700

Patent

active

043507689

ABSTRACT:
A method and apparatus for preparing a liquid suspension of single, independent tissue cells for, e.g., subsequent cell proliferation as by in vitro culturing, is disclosed. The method involves introducing the combination of subdivided tissue and liquid medium into a chamber defined in part by a foraminous wall portion, and contracting the chamber to force the tissue through the foraminous wall, thereby further subdividing the tissue. An apparatus embodiment includes the combination of confining means for confining a liquid suspension of tissue; a screen; means supporting the screen; and pump means for passing the liquid suspension of tissue from the confining means back and forth through the screen.

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