Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1997-07-08
1999-02-16
Jones, W. Gary
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 5, 435 6, 435 912, 514 44, 536 231, 536 243, 536 2431, 536 2433, C07H 2104, A61K 4800, C12Q 168, C12P 1934
Patent
active
058722424
ABSTRACT:
Compositions and methods are provided for the modulation of ras expression. Oligonucleotides are provided which are targeted to nucleic acids encoding human ras. Oligonucleotides specifically hybridizable with mRNA encoding human H-ras, Ki-ras and N-ras are provided. Such oligonucleotides can be used for therapeutics and diagnostics as well as for research purposes. Methods are also disclosed for modulating ras gene expression in cells and tissues using the oligonucleotides provided, and for specific modulation of expression of activated ras. Methods for diagnosis, detection and treatment of conditions associated with ras are also disclosed.
REFERENCES:
patent: 4871838 (1989-10-01), Bos et al.
patent: 5034506 (1991-07-01), Summerton et al.
patent: 5087617 (1992-02-01), Smith
patent: 5138045 (1992-08-01), Cook et al.
patent: 5218105 (1993-06-01), Cook et al.
patent: 5378825 (1995-01-01), Cook et al.
patent: 5459255 (1995-10-01), Cook et al.
Anfossi et al., "An Oligomer Complementary to c-myb-encoded mRNA inhibits proliferation of human myeloid leukemia cell lines", Proc. Natl. Acad. Sci. 1989, 86, 3379-3383.
Borer, P.N., Dengler, B., Tinoco, I., Jr., and Uhlenbeck, O.C., "Stability of Ribonucleic acid Double-stranded Helices", J. Mol. Biol., 1974, 86, 843-853.
Brown et al., "Modulation of ras Expression by Anti-sense, Nonionic Deoxyoligonucleotide Analogs", Oncogene Reasearch 1989, 4, 243-252.
Capon et al., "Complete nucleotide sequence of the T24 human bladder carcinoma oncogene and its normal homologue", Nature 302 1983, 33-37.
Chang et al., "Comparative inhibition of ras p21 protein synthesis with phosphorus-modified antisense oligonucleotides", Anti-Cancer Drug Design 1989, 4, 221-232.
Chang et al., "Antisense inhibition of ras p21 expression that is sensitive to a point mutation", Biochemistry 1991, 30, 8283-8286.
Chiang et al., "Antisense Oligonucleotides Inhibit Intercellular Adhesion Molecule 1 Expression by Two Distinct Mechanisms", J. Biol. Chem. 1991, 266:18162-18171.
De Mesmaeker et al., "Antisense Oligonucleotides", Acc. Chem. Res. 1995, 28:366-374.
Dignam et al., "Accurate transcription initiation by RNA polymerase II in a soluble extract from Isolated mammalian nuclei", Nucleic Acids Res. 1983, 11, 1475-1489.
Feramisco et al., "Transient reversion of ras oncogene-induced cell transformation by antibodies specific for amino acid 12 of ras protein", Nature 1985, 314, 639-642.
Gebeyehu, G., et al., "Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA", Nucl. Acids Res. 1987, 15:4513-4534.
Georges, R.N. et al., "Prevention of Orthotopic Human Lung Cancer Growth by Intratracheal Instillation of a Retroviral Antisense K-ras Construct", 1993, Cancer Research, 53, 1743-1746.
Greenberg, M.E., in Current Protocols in Molecular Biology, (F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.A. Smith, J.G. Seidman and K. Strahl, eds.), John Wiley and Sons, NY.
Hall et al., "Identification of transforming gene in two human sarcoma cell lines as a new member of a ras gene family located on chromosome 1", Nature 1983, 303: 396-400.
Hall and Brown, "Human N-ras: cDNA cloning and gene structure", Nucleic Acids Res. 1985, 13, 5255-5268.
Holt et al., "An Oligomer Complementary of c-myc mRNA Inhibits Proliferation of HL-60 Promyelocytic Cells and Induces Differentiation", Mol. Cell Biol. 1988, 8, 963-973.
Kabanov et al., "A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells", FEBS Lett. 1990, 259, 327-330.
Kahn et al., The c-K-ras gene and human cancer (review), Anticancer Res. 1987, 7, 639-652.
Kawasaki et al., "Uniformly Modified 2'-Deoxy-2'-fluoro Phosphorothioate Oligonucleotides as Nuclease-Resistant Antisense Compounds with High Affinity and Specificity for RNA Targets", J. Med. Chem. 1993, 36, 831-841.
Kingston, R.E., in Current Protocols in Molecular Biology, (F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.A. Smith, J.G. Seidman and K. Strahl, eds.), John Wiley and Sons, NY.
Kornberg, A., DNA Replication, W.H. Freeman & Co., San Francisco, 1980, pp. 75-77.
Lima et al., "Implication of RNA Structure on Antisense Oligonucleotide Hybridization Kinetics", Biochemistry 1992, 31, 12055-12061.
Letsinger et al., "Cholesteryl-conjugated oligonucleotides: Synthesis, properties, and activity as inhibitors of replication of human immunodeficiency virus in cell culture", Proc. Natl. Acad. Sci. USA 1989, 86, 6553-6556.
Mukhopadhyay, T. et al., "Specific Inhibition of K-ras Expression and Tumorigenicity of Lung Cancer Cells by Antisense RNA", (1991) Cancer Research 51, 1744-1748.
Martin, P., "Ein neuer Zugang zu 2'-O-Alkylribonucleosiden und Eigenschaften deren Oligonucleotide", Helv. Chim. Acta 1995, 78, 486-504.
Manoharan et al., "Cholic Acid-Oligonucleotide Conjugates for Antisense Applications", Bioorg. Med. Chem. Let. 1994, 4, 1053-1060.
Manoharan et al., "Introduction of a Lipophilic Thioether Tether in the Minor Groove of Nucleic Acids for Antisense Applications", Bioorg. Med. Chem. Let. 1993, 3, 2765-2770.
Manoharan et al., "Antisense Strategies", Ann. N.Y. Acad. Sci. 1992, 660, 306-309.
Manoharan et al., "Lipidic Nucleic Acids", Tetrahedron Lett. 1995, 36, 3651-3654.
Manoharan et al., "Oligonucleotides Conjugates: Alteration of the Pharmacokinetic Properties of Antisense Agents", Nucleosides & Nucleotides 1995, 14, 969-973.
McGrath, J.P. et al., "Structure and organization of the human Ki-ras proto-oncogene and a related processed pseudogene", Nature 1983, 304, 501-506.
Nielsen et al., "Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide", Science 1991, 254, 1497.
Oberhauser et al., "Effective incorporation of 2'-O-methyl-oligoribonucleotides into liposomes and enhanced cell association through modification with thiocholesterol", Nucl. Acids Res. 1992, 20, 533-538.
Owen et al., "Transcriptional activation of a conserved sequence element by ras requires a nuclear factor distinct from c-fos or c-jun", Proc. Natl. Acad. Sci. U.S.A. 1990, 87, 3866-3870.
Petersheim, M. and Turner, D.H., "Base Stacking and Base-Pairing Contributions to Helix Stability: Thermodynamics of Double-Helix Formation with CCGG, CCGGp, CCGGAp, ACCGGp, CCGGUp, and ACCGGUp", Biochemistry 1983, 22, 256-263.
Puglisi and Tinoco, "Absorbance Melting Curves of RNAs", Methods in Enzymol. 1989, 180, 304-325.
Reddy, P.E. et al., "A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene", Nature 1982, 300, 149-152.
Sanghvi et al., "Antisense oligodeoxynucleotides: synthesis; biophysical and biological evaluation of oligodeoxynucleotides containing modified pyrimidines", Nucl. Acids Res. 1993, 21, 3197-3203.
Sanghvi, Y.S., in Crooke, S.T. and Lebleu, B., eds., Antisense Research and Applications, CRC Press, Boca Raton, 1993, pp. 276-288.
Saison-Behmoaras, T. et al., "Short modified antisense oligonucleotides directed against Ha-ras point mutation induce selective cleavage of the mRNA and inhibit T24 cells proliferation", EMBO J. 1991, 10, 1111-1118.
Skorski, et al., "Growth Factor-dependent Inhibition of Normal Hematopoiesis by N-ras Antisense Oligodeoxynucleotides", J. Exp. Med., 1992, 175, 743-750.
Svinarchuk et al., "Inhibition of HIV proliferation in MT-4 cells by antisense oligonucleotide conjugated to lipophilic groups", Biochimie 1993, 75, 49-54.
Shea, "Synthesis, hybridization properties and antiviral activity of lipid-oligodeoxynucleotide conjugates", et al. Nucl. Acids Res. 1990, 18, 3777-3783.
Tidd et al., "Evaluation of N-ras oncogene anti-sense, sense and nonsense sequence methylphosphonate oligonucleotide analogues", Anti-Cancer Drug Design 1988, 3, 117-127.
Tabin, C.J. et al., "Mechanism of activation of a human oncogene", Nature 1982, 300, 143-149.
Taparowsky, E. et al., "Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change", Nature 1982, 300, 762-765.
Taparowsky et al., "
Cowsert Lex M.
Manoharan Muthiah
Monia Brett P.
Fredman Jeffrey
ISIS Pharmaceuticals Inc.
Jones W. Gary
LandOfFree
Antisense oligonucleotide inhibition of ras does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Antisense oligonucleotide inhibition of ras, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antisense oligonucleotide inhibition of ras will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2063454