DNA sequencing by mass spectrometry via exonuclease degradation

Details DNA sequencing by mass spectrometry via exonuclease degradation DNA sequencing by mass spectrometry via exonuclease degradation

1995-05-30

1999-02-16

Redding, David A.

Chemistry: molecular biology and microbiology

Apparatus

4352861, 4352872, 4352887, 4352873, 935 77, 935 86, 935 88, C12M 300

Patent

active

058720030

ABSTRACT:
Methods for determining the sequence of nucleic acids by cleaving the nucleic acid unilaterally from a first end with an exonuclease activity to sequentially release individual nucleotides, identifying each of the sequentially release nucleotides by mass spectrometry, and determining the sequence of the nucleic acid from the identified nucleotides are disclosed. The method is amenable to multiplexing for simulataneously determining more than one nuleic acid sequence.

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