Method of measuring enzyme reaction by Raman scattering

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, C12Q 126

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active

057416605

ABSTRACT:
As to reaction solutions in which the amount of each potassium ferricyanate, glucose oxidase and glucose substrate are set at a constant value while glucose concentrations are varied, intensity changes of peaks at a shift wavenumber of 2081 cm.sup.-1 in light scattering spectra are measured every 10 seconds, the maximum values of the intensity changes of the peaks at the respective glucose concentrations obtained, for obtaining a calibration curve from correlations between the maximum values of the reaction velocities and the glucose concentrations. The calibration curve is employed for carrying out quantitative measurement of an unknown sample.

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patent: 5481113 (1996-01-01), Dou et al.
Kitagawa et at (J. Biochem, vol. 84, No. 5, 1978, pp. 1245-1252).
"Resonance Raman Studies of Cytochrome Oxidase".
Adar et al (Biochimica et Biophysica Acta, vol. 502, 1978, pp. 80-86), Resonance Raman Spectra of Cytochrome Oxidase Evidence for Photoreduction by Laser Photons in Resonance with the Soret Band.

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