Morphogenic protein screening method

Details Morphogenic protein screening method Morphogenic protein screening method

1995-05-26

1998-04-21

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, C12Q 168, G01N 3353

Patent

active

057416419

ABSTRACT:
Disclosed is a method of screening candidate compounds for the ability to modulate the level of morphogenic protein in mammalian system. The method includes determining a parameter indicative of the level of production of a morphogenic in a cell culture known to produce the morphogen, incubating a candidate compound with the culture for a time sufficient to allow the compound to affect the production of the morphogenic protein, and then assaying the culture again to detect a change in the level of morphogenic protein production.

REFERENCES:
patent: 4089028 (1978-05-01), Housey et al.
patent: 4968590 (1990-11-01), Kuberasampath et al.
patent: 5011691 (1991-04-01), Oppermann et al.
Higgins, MD, "The Formation of Bone Under the Influence of Epithelium of the Urinary Tract," (Submitted for Publication, 1930); pp. 337-408.
Urist (1965), "Bone: Formation by Autoinduction," 150 Science pp. 893-899.
Needleman et al. (1970), "A General Method Applicable to the Search for Similarities in the Amino Acid Sequence of Two Proteins," 48 Journal of Molecular Biology; pp. 443-453.
Reddi et al. (1972), "Biochemical Sequences in the Transformation of Normal Fibroblasts in Adolescent Rats," 69 Pro. Natl. Acad. Sci. USA; pp. 1601-1605.
Dayhoff et al. (1978), "A Model of Evolutionary Change in Proteins," 5 Atlas of Protein Sequence and Structure, pp. 345-352.
Reddi (1981), "Cell Biology and Biochemistry of Endochondral Bone Development," 1 Coll. Res.; pp. 209-226.
Sampath et al. (1983), "Homology of Bone-Inductive Proteins from Human, Monkey, Bovine, and Rat Extracellular Matrix," 60 Proc. Natl. Acad. Sci. USA; pp. 6591-6595.
Heijne (1986), "A New Method for Predicting Signal Sequence Cleavage Sites," 14 Nucleic Acids Research; pp. 4683-4690.
Chomcxynski et al. (1987), Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate--Phenol--Chloroform Extraction, 162 Anal. Biochem 156-159.
Miller et al. (1987), "Phenotypic Modulation of the Swarm Rat Chondrosarcoma Induced by Morphogenetic Bone Matrix," 42 Cancer Research; pp. 3589-3594.
Weeks et al. (1987), "A Maternal MRNA Localized to the Vegetal Hemisphere in Xenopus Eggs Codes for a Growth Factor Related to TGF-B," 51 Cell; pp. 861-867.
Padgett et al. (1987), "A Transcript from a Drosophila Pattern Gene Predicts a Protein Homologous to the Transforming Growth Factor-B Family," 325 Nature; pp. 81-84.
Nemec et al. (1987), "The Cell Surface Hyaluronate Binding Sites of Invasive Human Bladder Carcinoma Cells," 149 Biochemical and Biophysical Research Communications; pp. 249-257.
Wang et al. (1988), "Purification and Characterization of Other Distinct Bone-Inducing Factors," 85 Proc. Natl. Acad. Sci. USA; pp. 9484-9488.
Rosen et al.; Wang et al. and Wozney et al., (1988) 42 Calcified Tissue Int. (Suppl.): A35 (136), A37 (146, 147) 3 Abstracts.
Wozney et al. (1988), "Novel Regulators of Bone Formation: Molecular Clones and Activities," 242 Science; pp. 1528-1534.
Rosen et al. (1989), "Purification and Molecular Cloning of a Novel Group of BMPS and Localization of BMP MRNA in Developing Bone," Connective Tissue Research; pp. 313-319.
Boyd (1989), "Examination of the Effects of Epidermal Growth Factor on the Production of Urokinase and the Expression of the Plasminogen Activator Receptor in a Human Colon Cancer Cell Line," 49 Cancer Research; pp. 2427-2432.
Lyons et al. (1989), "VGR-1, A Mammalian Gene Releated to Xenopus VG-1, is a Member of the Transforming Growth Factor B Gene Superfamily," 86 pp. 4554-4558.
Wozney (1989), "Bone Morphogenetic Proteins," 1 Progress in Growth Factor Research; pp. 267-280.
Ozkaynak et al. (1990), "OP-1 CDNA Encodes an Osteogenic Protein in the TGF-B Family," 9 The EMBO Journal; pp. 2085-2093.
Panganiban et al (1990), "Biochemical Characterization of the Drosophila dpp Protein, a Member of the Transforming Growth Factor B Family of Growth Factors," 10 Molecular and Cellular Biology; pp. 2669-2677.
Sampath, et al. (1990), "Bovine Osteogenic Protein Is Composed of Dimers of OP-1 and BMP-2A, Two Members of the Transforming Growth Factor-B Superfamily," 265 The Journal of Biological Chemistry; pp. 13198-13205.
Celeste et al. (1990), "Identification of Transforming Growth Factor B Family Members Present in Bone-Inductive Protein Purified from Bovine Bone," 87 Proc. Natl. Acad. Sci. USA; pp. 9843-9847.
Roson et al. (1990), "An Alternative Method for the Visualization of RNA in Formaldehyde Agarose Gels," 12 Focus; pp. 23-24.
Wang et al. (1990), "Recombinant Human Bone Morphogenetic Protein Induces Bone Formation," 87 Proc. Natl. Acad. Sci. USA; pp. 2220-2324.
Rosen et al., Celeste et al., UCLA Symposia on Molecular & Cellular Biology (1990), 2 Abstracts, Supplement 14E Journal of Cellular Biochemistry; p. 33 (004), 54 (105).
Katagiri et al. (1990), "The Non-Osteogenic Mouse Pluripotent Cell Line, C3H10T1/2, is Induced to Differentiate into Osteoblastic Cells By Recombinant Human Bone Morphogenetic Protein-2," 172 Biochemical and Biophysical Research Communications; pp. 295-299.
Wozney et al. (1990), "Growth Factors Influencing Bone Development," Suppl. 13 J. Cell Sci.; pp. 149-156.
Ozkaynak et al. (1991), "Murine Osteogenic Protein (OP-1): High Levels of MRNA in Kidney," 179 Biochemical and Biophysical Research Communications; pp. 116-123.
Wharton et al. (1991), "Drosophila 60A Gene, Another Transforming Growth Factor B Family Member, is Closely Related to Human Bone Morphogenetic Proteins," 88 Proc. Natl. Acad. Sci. USA; pp. 9214-9218.
Lee (1991), "Expression of Growth/Differentiation Factor 1 in the Nervous System: Conservation of a Bicistronic Structure," 88 Proc. Natl. Acad. Sci. USA; pp. 4250-4254.
Takuwa et al. (1991), "Bone Morphogenetic Protein-2 Stimulates Alkaline Phosphatase Activity and Collagen Synthesis in Cultured Osteoblastic Cells, MC3T3-E1," 174 Biochemical and Biophysical Research Communications; pp. 96-101.
Yamaguchi et al. (1991), "Recombinant Human Bone Morphogenetic Protein-2 Stimulates Osteoblastic Maturation and Inhibits Myogenic Differentation in Vitro," 113 The Journal Cell of Biology; pp. 681-687.
D'Allessandro et al., Keystone Symposia on Molecular Cellular Biology (1991), "Wound Repair," (Q105) The Journal of Cellular Biochemistry; p. 166.
Israel et al., Keystone Symposia on Molecular Cellular Biology (1991), "Wound Repair," (Q111) The Journal of Cellular Biochemistry; p. 168.
Thies et al. (1992), "Recombinant Human Bone Morphogenetic Protein-2 Induces Osteoblastic Differentiation in W-20-17 Stromal Cells," 130 Endocrinology; pp. 1318-1324.
Wozney (1992), "The Bone Morphogenetic Protein Family and Osteogenesis," 32 Molecular Reproduction and Development; pp. 154-156.
Rogers et al. (1992), "Bone Morphogenetic Proteins-2 and -4 are Involved in the Retinoic Acid-Induced Differentiation of Embryonal Carcinoma Cells," 3 Molecular biology of the Cell; pp. 189-196.
Rosen et al.; Celeste et al.; Wozney et al., Keystone Symposia on Molecular Cellular Biology (1992), "Growth and Differentiation Factors in Vertebrate Development," Suppl. 18F The Journal of Cellular Biochemistry; p. 103 (W513), 100 (W502), 76 (W026).
Israel et al. (1992), "Expression and Characterization of Bone Morphogenetic Protein-2 in Chinese Hamster Ovary Cells," 7 Growth Factors; pp. 139-150.
Hemmati-Brivanlou et al. (1992), "A Truncated Activin Receptor Inhibits Mseoderm Induction and Formation of Axial Structures in Xenopus Embryos," 359 Nature; pp. 609-614.
Padgett et al. (1993), "Human BMP Sequences Can Confer Normal Dorsal-Ventral Patterning in the Drosophila Embryo," 90 Proc. Natl. Acad. Sci. USA; pp. 2905-2909.
Piqueras et al. (1993), "Localization of Osteogenic Protein-1 (OP-1) mRNA and Protein Expression in Kidney," J. American Soc. Nephrology 4(3), 700(A).

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