Microtiter well for detecting nucleic acid

Details Microtiter well for detecting nucleic acid Microtiter well for detecting nucleic acid

1994-12-19

1998-04-21

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168, A61L 300

Patent

active

057416389

ABSTRACT:
A microtiter well in which a single stranded nucleic acid including a plurality of sequences specifically hybridizalbe with a target nucleic acid is immobilized is disclosed. The single stranded nucleic acid is derived from a phage or a phage-plasmid. The microtiter well enables a target nucleic acid to be specifically detected with a high sensitivity and high efficiency and further enables the detection procedure to be automated.

REFERENCES:
Cook et al., Nucl. Acid. Res. 16:4077-4095 (1988) "Synthesis and Hybridization of a Series . . . ".
Nagata et al., FEBS 183: 379-382 (1985) "Quantification of Picogram Levels of Specific DNA . . . ".
Rosmvssen et al., Analytical Biochem 198:138-142 (1991) "Covalent Immobilization of DNA . . . ".
Murray et al., Mol. Biochem. Parasitol 30:209-216 (1988) "Cloning and Characterization of a Species-spefic . . . ".
Urdea et al, Nucleic Acid Res 16: 4937-4956 (1988).

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