Method for producing antibodies and protein toxins in plant cell

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4353201, 435419, 435468, 536 241, 536 236, 536 235, C12N 1529, C12N 1581, C12N 1500

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061400750

ABSTRACT:
A method for producing antibodies and plant protein toxins in plant cells is described which includes the steps of providing a genetic construct to encode the secretable protein, delivering copies of the construct into a liquid suspension culture of tobacco cells, selecting for cells that have acquired the genetic construct, and allowing the desired protein to accumulate in the liquid media in which the plant cells are grown, and isolating the desired protein away from the tobacco cells.

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