DNA sequencing by mass spectrometry via exonuclease degradation

Details DNA sequencing by mass spectrometry via exonuclease degradation DNA sequencing by mass spectrometry via exonuclease degradation

1998-09-25

2000-10-31

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 253, 422 681, C12Q 168

Patent

active

06140053&

ABSTRACT:
The invention provides fast and highly accurate mass spectrometer based processes for directly sequencing a target nucleic acid (or fragments generated from the target nucleic acid), which by means of protection, specificity of enzymatic activity, or immobilization, are unilaterally degraded in a stepwise manner via exonuclease digestion and the nucleotides, derivatives or truncated sequences detected by mass spectrometry.

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