Methods for recombinant microbial production of fusion proteins

Details Methods for recombinant microbial production of fusion proteins Methods for recombinant microbial production of fusion proteins

1996-03-22

1998-12-22

Spector, Lorraine

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 698, 4352523, 43525233, 530350, 536 234, C12M 1562

Patent

active

058518029

ABSTRACT:
The present invention relates to methods and materials for the recombinant microbial production of fusion proteins and peptides derived from or based on Domain I (amino acids 17-45), Domain II (amino acids 65-99) and Domain III (amino acids 142-169) of bactericidal/permeability-increasing protein (BPI).

REFERENCES:
patent: 5206154 (1993-04-01), Lai et al.
patent: 5284756 (1994-02-01), Grinna et al.
patent: 5416202 (1995-05-01), Bernhard et al.
patent: 5627153 (1997-05-01), Little, II et al.
Qi et al. The region around residue 115 of the human bactericidal/permeability increasing protein is not involved in lipopolysccharide binding or bactericidal activity: chemical synthesis and expression of a gene coding for the active domain and characte, 1994.
Callaway et al., "Modification of the C Terminus of Cecropin Is Essential for Broad-Spectrum Antimicrobial Activity," Antimicrob. Agents & Chemo., 37(8):1614-1619 (1993).
Dykes et al., "Expression of aerial natriuretic factor as a cleavable fusion protein with chloramphenicol acetyltransferase in Escheria coli," Eur. J. Biochem., 174:411-416 (1988).
Elsbach and Weiss, "Oxygen-Independent Bactericidal Systems of Polymorphonuclear Leukocytes," in Advances in Inflammation Research, ed. G. Weissmann, vol. 2, pp. 95-113 Raven Press (1981).
Elsbach and Weiss, "Oxygen-Independent Antimicrobial Systems of Phagocytes," in Inflammation: Basic Principles and Clinical Correlates, eds. Gallin et al., Chapter 30, Raven Press, Ltd. (1992).
Elsbach et al., "Separation and Purification of a Potent Bactericidal/Permeability-Increasing Protein and a Closely Associated Phospholipase A.sub.2 from Rabbit Polymorphonuclear Leukocytes," J. Biol. Chem., 254:11000-11009 (Nov. 10, 1979).
Gazzano-Santoro et al., "High-Affinity Binding of the Bactericidal/Permeability-Increasing Protein and a Recombinant Amino-Terminal Fragment to the Lipid A Region of Lipopolysaccharide," Infect. Immun., 60:4754-4761 (Nov. 1992).
Gramm et al., "A Novel Approach for High Level Production of a Recombinant Human Parathryiod Hormone Fragment in Escheria coli," Bio/Technology, 12:1017 (Oct. 12, 1994).
Gray et al., "Cloning of the cDNA of a Human Neutrophil Bactericidal Protein," J. Biol. Chem., 264:9505-9509 (Jun. 5, 1989).
In't Veld et al., "Effects of the Bactericidal/Permeability-Increasing Proein of Polymorphonuclear Leukocytes on Isolated Bacterial Cytoplasmic Membrane Vesicles," Infection and Immunity 56:1203-1208 (1988).
Kempe et al., "Multiple-copy genes: production and modification of monomeric peptides from large multimeric fusion proteins," Gene, 39:239-245 (1985).
Kuliopulos and Walsh, "Production, Purification, and Cleavage of Tandem Repeats of Recombinant Peptides," J. Am. Chem. Soc., 116:4599-4607 (1994).
Lennick et al., "High-level expression of .alpha.-human atrial natriuretic peptide from multiple joined genes in Escheria coli," Gene, 61:103-112 (1987).
Mannion et al., "Separation of Sublethal and Lethal Effects of Polymorphonuclear Leukocytes on Escherichia coli," J. Clin. Invest. 86:631-641 (1990).
Mannion et al., "Separation of Sublethal and Lethal Effects of Bactericidal/Permeability Increasing Protein on Escherichia coli," J. Clin. Invest., 85:853-860 (Mar. 1990).
Ooi et al., "A 25-kDa NH.sub.2 -terminal Fragment Carries All the Antibacterial Activities of the Human Neutrophil 60-kDa Bactericidal/Permeability-increasing Protein," J. Bio. Chem., 262:14891-14894 (1987).
Ooi et al., "Endotoxin-neutralizing Properties of the 25 kD N-Terminal Fragment and a Newly Isolated 30 kD C-Terminal Fragment of the 55-60 kD Bactericidal/Permeability-Increasing Protein of Human Neutrophils," J. Exp. Med., 174:649-655 (Sep. 1991).
Piers et al., "Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria," Gene, 134:7-13 (1993).
Ray et al., "Production of Recombinant Salmon Calcitonin by In Vitro Amidation of an Escheria coli Produced Precursor Peptide," Bio/ Technology, 11:64 (Jan. 1993).
Schellenberger et al., "Peptide production by a combination of gene expression, chemical synthesis, and protease-catalyzed conversion," Int. J. Peptide Protein Res., 41:326-332 (1993).
Shen, "Multiple joined genes prevent product degradation in Escheria coli," Proc. Natl. Acad. Sci. USA, 81:4627-4631 (Aug. 1984).
Weiss et al., "The Role of Lipopolysaccharides in the Action of the Bactericidal/Permeability-Increasing Neutrophil Protein on the Bacterial Envelope," J. Immunol. 132:3109-3115 (1984).
Weiss et al., "Resistance of Gram-negative Bacteria to Purified Bactericidal Leukocyte Proteins," J. Clin. Invest. 65:619-628 (1980).
Weiss and Olsson, "Cellular and Subcellular Localization of the Bactericidal/Permeability-Increasing Protein of Neutrophils," Blood, 69:652-659 (Feb. 1987).
Weissmann, vol. 2, pp. 95-113 Raven Press (1981).

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