Compositions and methods for genetic transformation of pineapple

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Involving particle-mediated transfecion

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435 691, 435419, 435430, 4354301, 800283, 800286, 800288, 800298, C12N 504, C12N 1582, C12N 1590, A01H 500

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060910038

ABSTRACT:
The present invention provides methods for the production of transgenic pineapple-like totipotent bodies, and in particular, transgenic pineapple-like callus and transgenic pineapple-like protocorm-like bodies. Also provided by this invention are methods for the production of transgenic plants from transgenic totipotent bodies which include transgenic pineapple-like callus and protocorm-like bodies. The invention additionally provides transgenic pineapple-like plants which may be genetically engineered to exhibit resistance to pests and disease and to exhibit improved qualities. The invention further provides improved methods for the maintenance of pineapple-like protocorm-like bodies in culture. These improved methods are useful for reducing the time, cost, and labor involved in selecting stably transformed pineapple-like protocorm-like bodies.

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