Nucleic acid encoding helicobacter pylori enolase

Details Nucleic acid encoding helicobacter pylori enolase Nucleic acid encoding helicobacter pylori enolase

1995-05-22

1997-12-30

Wax, Robert A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

4353201, 4352523, C07H 2104, C12N 1563, C12N 121

Patent

active

057032190

ABSTRACT:
An isolated nucleic acid encoding the Helicobacter pylori recombinase is provided. Also provided is an isolated nucleic acid that hybridizes with the recombinase-encoding nucleic acid under stringent conditions and has at least 70% complementarity with the segment of the nucleic acid of SEQ ID NO:1 to which it hybridizes. Also provided is a mutant strain of H. pylori that does not express a functional recombinase (recA.sup.- mutant). An isolated nucleic acid encoding the Helicobacter pylori enolase is provided. Also provided is an isolated nucleic acid that hybridizes with the nucleic acid that encodes a H. pylori recombinase under stringent conditions and has at least 70% complementarity with the segment of the nucleic acid of SEQ ID NO:9 to which it hybridizes. Also provided is a mutant strain of H. pylori that does not express a functional enolase (eno.sup.- mutant). A method is provided for immunizing a subject against infection by H. pylori by administering to the subject an immunogenic amount of mutant H. pylori in a carrier for the mutant.

REFERENCES:
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Labigne-Roussel et al. J. Bacteriol. 170(4):1704-1708, Apr. 1988.
Perez-Perez and Blaser Infect. Immun. 55(5):1256-1263, May 1987.
Keener et al. J. Bact 160(1):153-160, Oct. 1984.
Leyva-Vazquez, M.A. et al. (1994) "Cloning and nucleotide sequences of the genes encoding thriose phosphate isomerase, phosphoglycerate mutase, and enolase from Bacillus subtilis" J. Bact. 176(13):3903-3910, Jul. 1994.
Burnett, M.E. et al. (1992) "Molecular characterization of the Zymomonas mobilis enoase (eno) gene" J. Bact. 174(20):6548-6553, Oct. 1992.

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