Process for amplifying nucleic acid sequences

Details Process for amplifying nucleic acid sequences Process for amplifying nucleic acid sequences

1985-10-25

1987-07-28

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4351773, 435317, 536 27, 536 28, 536 29, 935 17, 935 18, 935 16, C12P 1934, C12N 1500, C12N 100, C07H 2104, C07H 2102

Patent

active

046832025

ABSTRACT:
The present invention is directed to a process for amplifying any desired specific nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, and extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.

REFERENCES:
Gaubatz et al, "Strategies for Constructing Complementary DNA for Cloning", J. Theor. Biol. 95: 679 (1982).
Caton and Robertson, Nucleic Acids Research, vol. 7, pp. 1445-1456 (1979).
Rossi et al., J. Biol. Chem., 257, 9226-9229 (1982).

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