Enzymatic purification process

Details Enzymatic purification process Enzymatic purification process

1988-06-03

1991-04-09

Rosen, Sam

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

435262, 435267, 435268, 435272, C12N 108

Patent

active

050064720

ABSTRACT:
Controlled enzymatic treatment may be used to selectively degrade undesirable contaminants to a size or charge range which can be more readily removed by subsequent separation steps. Treatment is especially useful for purifying rDNA or monoclonal antibody culture products by using nuclease enzyme treatment to degrade undesirable residual nucleic acids to a molecular size or charge range sufficiently different from the product to be purified so that this difference can be exploited in a subsequent purification step (e.g. precipitation, size exclusion chromatography or ion exchange chromatography). The nuclease enzyme treatment is done in the presence of a detergent.

REFERENCES:
patent: 3496065 (1970-02-01), Russell
patent: 3867255 (1975-02-01), Newell et al.
patent: 3887431 (1975-06-01), Robbins et al.
patent: 4361587 (1982-11-01), Brule et al.
patent: 4729958 (1988-03-01), Drozd et al.

Affiliated with

Also associated with

Rating

Enzymatic purification process does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic purification process, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic purification process will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2034201