TMB formulation for soluble and precipitable HRP-ELISA

Details TMB formulation for soluble and precipitable HRP-ELISA TMB formulation for soluble and precipitable HRP-ELISA

1989-11-03

1991-04-09

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

534808, 435 6, 435 28, 435863, 435967, C12G 100, G01N 3353, C12Q 168, C12Q 128

Patent

active

050064614

ABSTRACT:
Solvent systems for use in increasing the solubility of 3,3',5,5'-tetramethylbenzidine (TMB) and for making a permanent color record of the results of ELISAs using HRP have been developed. In the improved solvent system containing povidone, 1-ethenyl-2-pyrrolidinone polymers, TMB can be dissolved in a very small quantity of solvent, up to a concentration of 100 to 200 mM, which can then be mixed directly into an aqueous buffer without precipitating the TMB. In the solvent system for making a permanent record, alginic acid (AA), methyl vinyl ether/maleic anhydride copolymer (MVE/MAC), dextran sulfate (DS) and/or carrageenan are added to the aqueous buffer solution, with or without povidone, to form a colored precipitate with the reaction product.

REFERENCES:
patent: 4789630 (1988-12-01), Bloch et al.
Sax, N. I., et al., Hawley's Condensed Chemical Dictionary Van Nostrand Reinhold Co., Inc. 1987, pp. 34, 227, 1224, 1225, 725.

Affiliated with

Also associated with

Rating

TMB formulation for soluble and precipitable HRP-ELISA does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with TMB formulation for soluble and precipitable HRP-ELISA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and TMB formulation for soluble and precipitable HRP-ELISA will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2034160