Use of organic buffers to reduce dehydroascorbic acid interferen

Details Use of organic buffers to reduce dehydroascorbic acid interferen Use of organic buffers to reduce dehydroascorbic acid interferen

1986-09-04

1989-12-05

Benson, Robert

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 31, 435 38, 435 39, 435805, 435810, 436 1, 436 63, 436 93, 436164, 436169, 436170, 436171, 436172, 436805, 436904, 422 56, 422 61, C12Q 104

Patent

active

048852406

ABSTRACT:
The interference of dehydroascorbic acid, an oxidation product of ascorbic acid, in analytical methods is reduced with the use of certain nitrogen-containing organic buffers. Dehydroascorbic acid tends to cause premature reduction of reducible compounds which are used to provide detectable species in the presence of an analyte. These organic buffers, as opposed to inorganic buffers, prevent premature reduction of the reducible compounds. An analytical method can be carried out in solution or in a dry analytical element.

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