Production of Pseudomonas XA cells containing indolyl-3-alkane a

Details Production of Pseudomonas XA cells containing indolyl-3-alkane a Production of Pseudomonas XA cells containing indolyl-3-alkane a

1990-05-23

1993-09-14

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435176, 435178, 435180, 435189, 435195, 435259, 435802, 435814, 435818, 435874, C12N 120, C12N 902, C12N 914

Patent

active

052448070

ABSTRACT:
A culture of Pseudomonas XA cells containing indolyl-3-alkane alpha-hydroxylase(INDH) is produced by aerobic batch fermentation in a culture medium. The culture medium is in a vessel having a means for adjusting agitation rate. During culturing, the cells go through a logarithmic phase and a stationary phase. Oxygen concentration is measured in the culture medium at the beginning of the process to determine a first oxygen concentration. Agitation rate is adjusted to produce a second oxygen concentration in the culture medium of about two to about seven percent of the first oxygen concentration for a time period of about two to about seven hours which ends at the beginning of the stationary phase. An INDH having three subunits of different molecular weight is isolated from the cells. The INDH is stable, free from endotoxin-mediated side effects and has sufficient specific activity to be useful for depletion of tryptophan from aqueous solution. During use, the INDH may be in immobilized form.

REFERENCES:
patent: 4431428 (1984-02-01), Schwer
patent: 4438198 (1984-03-01), Schmer
patent: 4955857 (1990-09-01), Shettigar
patent: 4956290 (1990-09-01), Harrison, Jr. et al.
Roberts et al., The Journal of Biological Chemistry, vol. 252, No. 8, 1977, pp. 2640-2647.
Schmer, et al., Biochimica et Biophysica Acta, vol. 527, 1978, pp. 264-271.
Noda et al., J. Biol. Chem., 252:4413-4415 (1977).
Rosenfeld et al., J. Biol. Chem., 252:6970-6973 (1977).
Schmer et al., "Plasma Separation and Plasma Fractionation", pp. 284-297, Karger, Basel (1983).
Takai et al., Meth. in Enzymol., 142:195-217 (1987).

Affiliated with

Also associated with

Rating

Production of Pseudomonas XA cells containing indolyl-3-alkane a does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Production of Pseudomonas XA cells containing indolyl-3-alkane a, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of Pseudomonas XA cells containing indolyl-3-alkane a will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2025967