Method of regenerating soybeans from cultured soybean cotyledona

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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43524048, 43524049, 43524054, C12N 500

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049923757

ABSTRACT:
A method for regenerating soybean plants from cotyledonary nodes is disclosed. Soybean seeds are germinated on nutrient medium containing a cytokinin to produce a donor plant. The cotyledonary nodes of the donor plant are excised and divided into pieces. The cotyledonary node pieces are cultured on nutrient medium containing a cytokinin until callus tissue develops which contains shoots. The shoots are removed and rooted on nutrient medium free of exogenous hormone to form a plantlet.

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