Method for the determination of hemoglobin in trace amounts

Details Method for the determination of hemoglobin in trace amounts Method for the determination of hemoglobin in trace amounts

1977-05-31

1978-03-07

Serwin, R.E.

Chemistry: physical processes

Physical processes

Crystallization

252408, G01N 3316

Patent

active

040777723

ABSTRACT:
An improved technique for determining the presence of hemoglobin, having increased sensitivity, is provided by bringing the material to be tested into contact with an acidic solution of tetramethylbenzidine and hydrogen peroxide to form a mixture, allowing said mixture to stand for a predetermined period of time, and thereafter examining the mixture for the presence of a colored complex indicative of the presence of hemoglobin. Organic acid is present in a concentration generally of at least 30 percent by volume, to provide a pH of no more than about 2.

REFERENCES:
patent: 3663175 (1972-05-01), Depositar
patent: 3677707 (1972-07-01), Pride et al.
patent: 3975161 (1976-08-01), Svoboda et al.
patent: 4017261 (1977-04-01), Svoboda et al.
Crosby et al., Blood 11:380 (1956).
Holland et al., Tetrahedron 30:3299 (1974).

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