Spectrofluorometric apparatus for obtaining spectral image infor

Radiant energy – Luminophor irradiation

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356346, G01N 2164

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active

055043364

ABSTRACT:
A spectrofluorometric apparatus for obtaining spectral image information comprises a stimulating ray source, which produces a laser beam serving as stimulating rays, and a scanning device, which deflects the laser beam in two directions and causes the deflected laser beam to scan a sample. An optical system separates the fluorescence, which has been produced by the sample when the sample is exposed to the laser beam, from the optical path of the laser beam. A Fourier spectrometric system causes interference to occur with the fluorescence, which has been separated by the optical system from the optical path of the laser beam. The Fourier spectrometric system detects the brightness and darkness of the fluorescence, which are due to the interference, as a detection signal and carries out Fourier transformation on the detection signal. The spectrum of the fluorescence is thus measured for each point on the sample, and two-dimensional spectral image information of the sample is thereby obtained.

REFERENCES:
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patent: 4937457 (1990-06-01), Mitchell
patent: 4983041 (1991-01-01), Inaba
patent: 5117466 (1992-05-01), Buican et al.
patent: 5208651 (1993-05-01), Buican
patent: 5377003 (1994-12-01), Lewis et al.
"Multiplexed Spectral Imaging Technique--Fourier Transform Spectral Imaging--", Kazuyoshi ITOH; on The Review of Laser Engineering (Laser Kenkyu); vol. 15, No. 10, Oct. 1987, pp. 21-30.

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