Method for peptide C-terminal fragment sequence analysis and app

Details Method for peptide C-terminal fragment sequence analysis and app Method for peptide C-terminal fragment sequence analysis and app

1993-10-15

1995-11-28

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435174, 435180, 435212, 4352873, 4352877, 4352881, 4352971, 4352891, 436 86, 436 89, 436 90, 436162, 436178, 436810, 530333, 530334, 422101, 422102, C12Q 100, C12N 1100, C12M 140

Patent

active

054707030

ABSTRACT:
The present invention relates to a method for peptide C-terminal fragment sequence analysis, in which the fragment collection is carried out on an allylamine group-derivatized polymer membrane or on allylamine group-derivatized glass fiber filter paper; the collected C-terminal fragment is immobilized thereon using a water-soluble carbodiimide etc.; and the obtained immobilized product is subjected directly to amino acid sequence analysis. The present invention also relates to an apparatus for collecting a peptide fragment. According to the method of the present invention, peptides which are rich in hydrophobic groups in their C-terminus and are therefore difficult to trap with polyvalent ion carriers, currently used in the gas-phase sequencer, can be completely analyzed up to their C-terminus. Also, amino acid sequence analysis can be made even when the amount of C-terminal fragments is very small. In addition, since collection and immobilization of fragments can be done in the same bottle, the risks of contamination and mechanical loss are very low.

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