Whole blood sample preparation for polymerase chain reaction usi

Details Whole blood sample preparation for polymerase chain reaction usi Whole blood sample preparation for polymerase chain reaction usi

1996-03-12

1997-12-30

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 912, 435 6, 536 243, 536 2432, 424534, C12Q 170, C12P 1934, C07H 2104

Patent

active

057028842

ABSTRACT:
Leucocytes can be quickly and selectively separated from erythrocytes by subjecting a whole blood sample to a series of steps including lysing the erythrocytes and washing the remaining leucocytes with a solution containing ammonium chloride and a carboxylic acid or a metal carboxylate. The resulting white blood cells can be readily lysed and subjected to polymerase chain reaction to amplify and detect a target nucleic acid. The test kit useful in practicing the amplification method includes a labeled primer, a PCR reagent and a reagent mixture containing ammonium chloride and a carboxylic acid or a metal carboxylate for sample preparation.

REFERENCES:
patent: 4407942 (1983-10-01), Birnboim
patent: 4683195 (1987-07-01), Mullis
patent: 5300635 (1994-04-01), Macfarlane

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