Oligonucleotides with selectably cleavable and/or abasic sites

Details Oligonucleotides with selectably cleavable and/or abasic sites Oligonucleotides with selectably cleavable and/or abasic sites

1991-07-24

1994-11-22

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2431, 536 2432, 435 6, C07H 2104, C07H 2100

Patent

active

053670663

ABSTRACT:
A modified polynucleotide containing at least one cleavable or abasic site as shown below. ##STR1## DNA.sub.1 is a first segment of DNA; DNA.sub.2 is a second segment of DNA; and R.sub.m is C.sub.1 to C.sub.16 alkylene or an oxytheylene oligomer --(CH.sub.2 CH.sub.2 O).sub.z -- where z is an interger in the range of 1 to 16 inclusive, and R.sub.n is selected from the group consisting of ##STR2## Such polynucleotides are useful in solid phase hybridizations because they permit the release of a label from the solid support after the hybridization reaction.

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