Method for lysing liposomes using polyethyleneglycol monononylph

Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Preparation composition

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436533, 436800, 436829, 436 71, 436172, 436 8, G01N 33543, G01N 33546

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053668952

ABSTRACT:
A method for rapidly lysing liposomes having transition temperatures in the range of 35.degree. to 65.degree. C. is provided. Such liposomes are treated with a surfactant including ##STR1## wherein x represents an average of 9 or 12. The method is applicable to fluorescence immunoassay procedures.

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Makarova, T. V. et al. "The investigation of high-temperature transition in egg yolk lecithin liposomes", Biol. Nauki (Moscow), (5), 23-31, 1988, (English translation).
M. Sila et al., "Effects of Triton X-100 Concentration and Incubation Temperature On Carboxyfluorescein Release From Multimellar Liposomes," Biochimica et Biophysica Acta 859 (1986), pp. 165-170.
Ethonic.TM. Alcohol Ethoxylates, Technical Bulletin and Product Bulletin.

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