Process for the production of a viable cell culture infected by

Details Process for the production of a viable cell culture infected by Process for the production of a viable cell culture infected by

1994-02-02

1996-12-17

Sayala, Chhaya D.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435239, 4352402, 43524021, 4352403, 43524031, C12N 502, C12N 701

Patent

active

055852629

DESCRIPTION:

BRIEF SUMMARY
BACKGROUND OF THE INVENTION

The present invention relates to the production and maintenance of a viable cell culture or line infected by the virus associated with multiple sclerosis (MS). Such a culture in fact has the advantage of constituting a biological material which expresses the activity of the virus associated with MS, and as a result can be used for various experimental aims, in particular for tests for identification and characterization of the virus, but also for clinical or therapeutic aims.
"Viable culture" is understood as meaning any culture which keeps infected cells alive such that the virus associated with MS is expressed and which preserves the mitotic potency of said cells, in particular during culture of the latter by successive passages.
The present invention relates to a process for in vitro culture of cells infected by a virus present in individuals suffering from multiple sclerosis and to the infected cell lines thus obtained.


DESCRIPTION OF THE PRIOR ART

Multiple sclerosis (MS) is a demyelinizing disease of the central nervous system (CNS) which has been suspected for several years of being associated with a virus, although the causal agent has still not been determined with certainty.
Several works have supported this hypothesis of a viral etiology of the disease, but none of the known viruses tested have proven to be the casual agent sought.
Consequently, the observation in patients suffering from multiple sclerosis of phenomena comparable to an autoimmunity reaction has led to an "essential" autoimmune etiological hypothesis (Lisak R. P., Zweiman B. New Engl. J. Med 1977; 297, 850-853, and Lassmann H. and Wisniewski H. M. Arch. Neurol. 1979; 36, 490-497). However, this autoimmunity directed against certain components of the central nervous system has proven to be not very specific to MS and frequent in inflammations of the CNS which may or may not be associated with an infection, as has been demonstrated by Hirayama M. et al. (Neurology 1986; 36, 276-8) Kenneth G. Warren et al. (Annals of Neurology 1986; 20, 20-25), Suzumura A. et al. (Journal of Neuroimmunology 1986; 11, 137-47) and Tourtelotte W. et al. (Journal of Neurochemistry 1986; 46, 1086-93). Moreover, as E. J. Field noted (The Lancet 1989; 1, 1272), none of the immunosuppressive therapeutic agents has achieved decisive results against MS.
One hypothesis has been put forward, according to which a retrovirus is said to be the cause of the disease. The discovery by A. Gessain et al. (J. Infect. Disease 1988; 1226-1234) of neurological syndromes associated with the HTLV-1 virus, known at the start as the agent of T-cell leukemia in adults, has led several authors such as H. Koprowski et al. (Nature 1985; 318, 154), M. Ohta et al. (J. Immunol. 1986; 137, 3440), E. P. Reddy et al. (Science 1989; 243, 529), S. J. Greenberg et al. (Proc. Natl. Acad. Sci. USA 1989; 86, 2878), J. H. Richardson et al. (Science 1989; 246, 821), S. L. Hauser et al. (Nature 1986; 322, 176) and A. Karpas et al. (Nature 1986; 322, 177) to investigate an involvement of this human retrovirus in MS, although without success or with results which suggest cross-reactions.
There is moreover an animal model which is very close to MS and is induced by a retrovirus: the MAEDIVISNA virus in sheep. It is known that natural infection by this virus causes an ovine disease close to MS, as reported by Johnson R. T. (Rev. Infect. Dis. 1985; 7, 66-67), Narayan O. and Cork L. C. (Rev. Infect. Dis. 1985; 7, 89-98) and Nathanson N. et al. (Rev. Infect. Dis. 1985; 7, 75-82). Experimental infection of sheep by intraventricular inoculation of neurovirulent strains of the VISNA virus has established the responsibility of this virus in the origin of this demyelinizing infection in sheep. As explained by Nathanson N. et al. (Rev. Infect. Dis. 1985; 7, 75-82), Hoffman P. M. and Panitch H. S. ("Handbook of Clinical Neurology, 12; Viral diseases" R. R. McKendall, ed., Elsevier Science Publishing, Amsterdam, 1989, 453-466) and A. Haase (Nature 1986; 322, 130-136), it differs

REFERENCES:
patent: 4388298 (1983-06-01), Nazerian et al.
patent: 4647773 (1987-03-01), Gallo et al.
patent: 5225352 (1993-07-01), Zanetta et al.
R. Lisak et al., "In Vitro Cell-Mediated Immunity of Cerebrospinal-Fluid Lymphocytes To Myelin Basic Protein In Primary Demyelinating Diseases", The New England Journal of Medicine, vol. 297, vol. 16, Oct. 20, 1977, pp. 850-853.
C. R. Bangham et al., "PCR Analysis of DNA from Multiple Sclerosis Patients for the Presence of HTLV-I", Science, vol. 246, Nov. 10, 1989, pp. 821-824.
E. J. Field, "Immunological Treatment for Multiple Sclerosis", The Lancet, Jun. 3, 1989, p. 1272.
D. Johnson et al., "Quantitation of the Myelin-Associated Glycoprotein in Human Nervous Tissue from Controls and Multiple Sclerosis Patients", Journal of Neurochemistry, vol. 46, No. 4, 1986, pp. 1086-1093.
R. T. Johnson, "Nononcogenic Retrovirus Infections as Models for Chronic and Relapsing Human Diseases: Introduction", Reviews of Infectious Diseases, vol. 7, No. 1, Jan.-Feb. 1985, pp. 66-67.
S. L. Hauser et al., "Analysis of Human T-lymphotropic Virus Sequences in Multiple Sclerosis Tissue", Nature, vol. 322, Jul. 10, 1986, pp. 176-178.
H. Lassmann et al., "Chronic Relapsing Experimental Allergic Encephalomyelitis-Clinicopathological Comparison With Multiple Sclerosis", Arch Neuro, vol. 36, Aug. 1979, pp. 490-497.
A. T. Haase, "Pathogenesis of Lentivirus Infections", Nature, vol. 322, Jul. 10, 1986, pp. 130-136.
N. Nathanson et al., "Experimental Visna in Icelandic Sheep: The Prototype Lentiviral Infection", Reviews of Infectious Diseases, vol. 7, No. 1, Jan.-Feb. 1985, pp. 75-82.
S. Haahr et al., "Just Another Dubious Virus in Cells from a Patient with Multiple Sclerosis?", The Lancet, vol. 337, Apr. 6, 1991, pp. 863-864.
M. Ohta et al., "Sera from Patients with Multiple Sclerosis React with Human T Cell Lymphotropic Virus-I Gag Proteins But Not Env Proteins-Western Blotting Analysis", The Journal of Immunology, vol. 137, No. 11, Dec. 1, 1986, pp. 3440-3443.
H. Koprowski et al., "Multiple Sclerosis and Human T-cell Lymphotropic Retrovirusus", Nature, vol. 318, Nov. 14, 1985, pp. 154-160.
S. J. Greenberg et al., "Detection of Sequences Homologous to Human Retroviral DNA in Multiple Sclerosis by Gene Amplification", Proc. Natl. Acad. Sci. USA, vol. 86, Apr. 1989, pp. 2878-2882.
E. P. Reddy et al., "Amplification and Molecular Cloning of HTLV-I Sequences from DNA of Multiple Sclerosis Patients", Science, vol. 243, Jan. 27, 1989, pp. 529-533.
K. G. Warren et al.,"Diagnostic Value of Cerebrospinal Fluid Anti-Myelin Basic Protein in Patients with Multiple Sclerosis", Annals of Neurology, vol. 20, No. 1, Jul. 1986, pp. 20-25.
O. Narayan et al., "Lentiviral Diseases of Sheep and Goats: Chronic Pneumonia Leukoencephalomyelitis, and Arthritis", Reviews of Infectious Diseases, vol. 7, No. 1, Jan.-Feb. 1985, pp. 89-98.
A. Gessain et al.,"Intrathecal Synthesis of Antibodies to Human T Lymphotropic Virus Type I and the Presence of IgG Oligoclonal Bands in the Cerebrospinal Fluid of Patients with Endemic Tropical Spastic Paraparesis", The Journal of Infectious Diseases, vol. 157, No. 6, Jun. 1988, pp 1226-1234.
Bjare. Pharmac Ther. vol. 53 pp. 355-374. 1992.
ATCC Catalogue of Cell Lines and Hybridomas 6 ed. 1988 pp. 344-355.
H. Perron et al., "Leptomeningeal Cell Line from Multiple Sclerosis With Reverse Transcriptase Activity and Viral Particles", Biological Abstracts, vol. 89, No. 9, May 1, 1990.
H. Perron et al., "Isolation of Retrovirus from Patients With Multiple Sclerosis", The Lancet, vol. 337, No. 8745, Apr. 6, 1991, pp. 862-863.
C. Bosgiraud et al., "Ultrastructural Study on Visna Virus in Sheep Plexus Choroid Cells", Biological Abstracts, vol. 83, No. 7, 1987.
ATCC CRL 1700,1988, p. 165.

Affiliated with

Also associated with

Rating

Process for the production of a viable cell culture infected by does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for the production of a viable cell culture infected by , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the production of a viable cell culture infected by will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1990556