Wound healing

Details Wound healing Wound healing

1994-11-22

1997-08-26

Stone, Jacqueline M.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

435 41, 4351723, 4352401, 4352402, 43524021, 43524023, 514 2, A61K 4800, C12N 1500

Patent

active

056611323

ABSTRACT:
A DNA molecule encoding a secretable mature epidermal growth factor (EGF) polypeptide is delivered to a skin wound. The cells that take up the recombinant DNA construct express soluble EGF that is secreted into surrounding fluid. The presence of the EGF accelerates, by a statistically significant amount, the healing time of a wound treated in this manner.
The DNA molecule can be a genetic construction that expresses an EGF encoding portion that differs from the naturally occurring EGF precursor gene in that the only coding region retained from the precursor gene is that of the mature EGF polypeptide. Amino-terminal EGF-like repeats and the carboxy-terminal hydrophobic sequence that anchors natural EGF to the cell membrane are not present in the genetic construction.

REFERENCES:
patent: 3026874 (1962-03-01), Stevens
patent: 3288140 (1966-11-01), McCarthy
patent: 3580254 (1971-05-01), Stuart
patent: 3814097 (1974-06-01), Ganderson et al.
patent: 4304866 (1981-12-01), Green et al.
patent: 4767746 (1988-08-01), Catsimpoolas et al.
patent: 4788971 (1988-12-01), Quisno
patent: 4847193 (1989-07-01), Richards et al.
patent: 4868116 (1989-09-01), Morgan et al.
patent: 4944948 (1990-07-01), Uster et al.
patent: 4945056 (1990-07-01), Sanford et al.
patent: 4980286 (1990-12-01), Morgan et al.
patent: 5152757 (1992-10-01), Eriksson
patent: 5262128 (1993-11-01), Leighton et al.
Andree, C., et al., "In Vivo Transfer and Expression of a Human Epidermal Growth Factor Gene Accelerates Wound Repair," Proc. Natl. Acad. Sci. USA, 91:12188-12192 (1994).
Breuing, et al., "Healing of Partial Thickness Porcine Skin Wounds in a Liquid Environment," Journal of Surgical Research 52:50-58 (1992).
Eriksson, et al., "In Vivo Cell Culture Accelerates Reepithelialization," Surgical Forum XLII:670-673 (1991).
Garlick et al., "Retrovirus-Mediated Transduction of Cultured Epidermal Keratinocytes," Journal of Investigative Dermatology 97:824-829 (1991).
Juni, et al., "Controlled Drug Permeation. II. Comparative Permeability and Stability of Butamben and Benzocaine," Chem. Pharm. Bull. 25:1098-1100 (1977).
Kaufman, et al., "Topical Oxygen and Burn Wound Healing: A Review," Burns 9:169-173 (1983).
Majumdar, A., and P. Stroeve, "Diffusion of Local Anaesthetics Through Liquid Membranes," (1986).
Morgan et al., "Expression of an Exogenous Growth Hormone Gene by Transplantable Human Epidermal Cells," Science 237:1476-1479 (1987).
Rheinwald and Green, "Epidermal Keratinocytes: The Formation of Keratinizing Colonies from Single Cells," Cell, pp. 331-343 (1975).
Sporr, M.B., and A. B. Roberts, p. 86 in Peptide Growth Factors and Their Receptors I, Springer-Verlag, New York (1990).
Wilson et al., "Implantation of Vascular Grafts Lined with Genetically Modified Endothelial Cells," Science 244:1344-1346 (1989).
Wright, P. C., "Fundamentals of Acute Burn Care and Physical Therapy Management," Physical Therapy 64:1217-1231.
Marshall, Science, 269, 1995, 1050-1055.
Yang et al., PNAS, 87, 1990, 9568-9572.
Bell et al., Nuc. Acids Res., 14(21), 1986, 8427-8446.
Brown et al, J. Exp. Med., 163, 1986, 1319-1324.
Morgan et al, Science, 237, 1987, 1476-1479.
Flowers et al, PNAS, 87, 1990, 2349-2353.
Teumer et al, FASEB, 4, 1990, 3245-3250.

Affiliated with

Also associated with

Rating

Wound healing does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Wound healing, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Wound healing will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1988603