Modified transcription control unit and uses thereof

Details Modified transcription control unit and uses thereof Modified transcription control unit and uses thereof

1995-05-19

1997-08-26

Guzo, David

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 701, C12P 2100

Patent

active

056610025

ABSTRACT:
The present invention is a modified transcription control unit which contains the P2 enhancer of BK virus spaced closely to the upstream regulatory element of the major late promoter of adenovirus, the adenovirus-2 major late promoter, a poly-GT element positioned to stimulate said promoter and a DNA sequence containing the spliced tripartite leader sequence of adenovirus. The invention further comprises methods of using this modified transcription unit in cells expressing the adenovirus E1A gene product to produce useful substances. The invention further comprises methods to increase the levels of expression in stably transformed cells by performing a second transformation with a vector containing the modified transcription unit.

REFERENCES:
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Randal J. Kaufman, 1985, Proc. Natl. Acad. Sci. USA, 82, 689-693.
Logan, et al, 1984, Proc. Natl. Acad. Sci. USA, 81, 3655-3659.
Alonso-Caplen, et al, 1988 Journal of Virology, 62(5) 1606-1616.

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