Apparatus and method for measuring fluorescence intensities at a

Details Apparatus and method for measuring fluorescence intensities at a Apparatus and method for measuring fluorescence intensities at a

1991-07-16

1993-05-04

Turner, Samuel A.

Optics: measuring and testing

By particle light scattering

With photocell detection

356351, G01B 902

Patent

active

052086518

ABSTRACT:
Apparatus and method for measuring intensities at a plurality of wavelengths and lifetimes. A source of multiple-wavelength electromagnetic radiation is passed through a first interferometer modulated at a first frequency, the output thereof being directed into a sample to be investigated. The light emitted from the sample as a result of the interaction thereof with the excitation radiation is directed into a second interferometer modulated at a second frequency, and the output detected and analyzed. In this manner excitation, emission, and lifetime information may be obtained for a multiplicity of fluorochomes in the sample.

REFERENCES:
patent: 3984190 (1976-10-01), Ballett et al.
patent: 4905169 (1990-02-01), Buican et al.
patent: 4983041 (1991-01-01), Inaba
patent: 4999013 (1991-03-01), Zoechbauer et al.
Tudor N. Buican, "Real-Time Fourier Transform Spectrometry for Fluorescence Imaging and Flow Cytometry", Bioimaging and Two-Dimensional Spectroscopy, Louis C. Smith, Ed. (SPIE, Los Angeles, CA, 1990), vol. 1205, pp. 126-133.

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