Methods, compositions and devices for growing human hematopoieti

Details Methods, compositions and devices for growing human hematopoieti Methods, compositions and devices for growing human hematopoieti

1994-12-01

1997-02-25

Ziska, Suzanne E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351721, 4352041, 435373, 435377, 435395, 435401, C12N 1500, C12N 500, C12M 300

Patent

active

056058225

ABSTRACT:
Methods, compositions and devices are provided for the growth of hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types is required for the successful reconstruction of hematopoietic tissue ex vivo. At least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. Means are provided for maintaining the stromal cells and hematopoietic cells separately, to allow for early removal of the hematopoietic cells.

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